A5601
 |  |  |
 | Immunoblotting Whole cell extract of A431 cells was separated on SDS-PAGE and blotted with Rabbit Anti-Arp1/Centractin (Cat. No. A5601). The antibody was developed with Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate. (Lane A) Antibody dilution 1:1,000; (Lane B) Antibody dilution 1:1,000 + 5 mg/mL, Arp1/Centractin immunizing peptide |
Image
Useful Links & Tools
- MSDS
- Certificate of Analysis
- A5601 - Datasheet (56 KB)
- A5601-blot (15 KB )
- Bulk Quote
- Similar Products
A5601
Sigma
Anti-Arp1α/Centractin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| MDL number: | MFCD04040182 |
Description
| Immunogen | synthetic peptide corresponding to the mid-region of human Arp1α/centractin (amino acids 222-240). This sequence is identical in mouse and dog Arp1α/centractin, highly conserved (89% identity) in the α-centractin isoform, and not found in known actin isoforms. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 45 kDa |
| species reactivity | human, rat, mouse |
| application(s) | immunoblotting: 1:1,000 using rat brain cytosol extract or whole cell extract of human epidermoid carcinoma A431 cell line. |
| indirect immunofluorescence: 1:100 using mouse fibroblasts NIH3T3 cell line | |
| microarray: suitable | |
| Gene Information | human ... ACTR1A(10121) |
| rat ... Actr1a(294010) | |
| mouse ... Actr1a(54130) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Robinson, R., and Snyder, J., Colocalization studies of Arp1 and p150Glued to spindle microtubules during mitosis: the effect of cytochalasin on the organization of microtubules and motor proteins in PtK1 cells. Cell Biol. Int. 30, 631-639, (2006) Abstract |
